Generator

Part:BBa_I766225:Design

Designed by: Nili Sommovilla   Group: iGEM07_UCSF   (2007-10-22)


WMTM1-EE under weak constitutive promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1167
    Illegal EcoRI site found at 1313
    Illegal XbaI site found at 93
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1167
    Illegal EcoRI site found at 1313
    Illegal NheI site found at 1608
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1167
    Illegal EcoRI site found at 1313
    Illegal BglII site found at 699
    Illegal BamHI site found at 2597
    Illegal XhoI site found at 611
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1167
    Illegal EcoRI site found at 1313
    Illegal XbaI site found at 93
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1167
    Illegal EcoRI site found at 1313
    Illegal XbaI site found at 93
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

used combinatorial cloning methods described in the UCSF iGEM 2007 wiki.



Source

This is a composite device; see individual parts for source

References